Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/04: Difference between revisions
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(Autocreate 2015/05/04 Entry for Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Prep for cell cultures</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Overview== | ==Overview== | ||
The Type IIS digestion/ligation followed by PCR didn't work out. I'm going to go ahead and grow up cultures from the BioBricks cloning of KAH57 & KAH104 and try a plasmid miniprep followed by digestion tomorrow. | |||
Cultures started: | |||
* KAH57_v0120 | |||
* KAH104_v0120 | |||
* pMaxGFP | |||
* BioBrick cloning attempt (3 colonies) | |||
For a total of 6 cultures. | |||
Latest revision as of 00:56, 27 September 2017
Prep for cell cultures | Main project page Previous entry Next entry |
OverviewThe Type IIS digestion/ligation followed by PCR didn't work out. I'm going to go ahead and grow up cultures from the BioBricks cloning of KAH57 & KAH104 and try a plasmid miniprep followed by digestion tomorrow. Cultures started:
For a total of 6 cultures.
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