IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/28: Difference between revisions

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(Autocreate 2014/07/28 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Monday 7/28==
* Insert content here...
*Started a seed of the seed of colony #3 from ligation plate #4.  
*Started a seed of the LacI promotor part (R0011) from Kylie
 
Set up a digestion of RBS+TesA to be run on a gel tomorrow
 
 
{| {{table}} border="1" align=center <!-- Digestion table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent (μL)
| bgcolor=#cfcfcf | Volume (μL)
|-
|| DNA(RBS+TesA plasmid) || 10.0 μL
|-
|| 10X fast digest buffer || 2.0
|-
|| dH<sub>2</sub>O || 6.0
|-
|| XbaI|| 1.0
|-
|| PstI|| 1.0
|-
|| &nbsp; || 20.0 μL --> 37°C/ ~overnight
|}




Latest revision as of 00:08, 27 September 2017

Project name Main project page
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Monday 7/28

  • Started a seed of the seed of colony #3 from ligation plate #4.
  • Started a seed of the LacI promotor part (R0011) from Kylie

Set up a digestion of RBS+TesA to be run on a gel tomorrow


Reagent (μL) Volume (μL)
DNA(RBS+TesA plasmid) 10.0 μL
10X fast digest buffer 2.0
dH2O 6.0
XbaI 1.0
PstI 1.0
  20.0 μL --> 37°C/ ~overnight


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