IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/29: Difference between revisions
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(Autocreate 2014/07/29 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. TesA+RBS = 650<br> 5. TesA+RBS = 650<br> 7. TesA+RBS = 650<br> 9. ETOH= 3650(ETOH backbone=2000)<br> | |||
| rowspan="7" | [[image:24.jpg|400px]] | |||
|- | |||
|| DNA(plasmid) || 3.0 μL | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| PSTI || 1.0 | |||
|- | |||
|| EcoRI || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 4.0 | |||
|- | |||
|| || 10.0 μL --> 37°C/ ~35 min. | |||
|} | |||
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Revision as of 14:27, 29 July 2014
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