IGEM:Cambridge/2008/Notebook/Voltage/2008/08/05: Difference between revisions

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==Kdp?==
==Kdp?==
* There were transformants from yesterday's transformation, but after Biobrick PCR the gel showed short fragments, suggesting self ligation of the vector.
* There were transformants from yesterday's transformation
 
[[Image:Happy_faces.jpg|frame|center|James celebrates apparent success]]
 
* After Biobrick PCR the gel showed short fragments, suggesting self ligation of the vector.
 
[[Image:Sad_face.jpg|thumb|center|150px|Ellis realises the sad truth]]
 
* Repeated digest and ligation protocol from the weekend, with EcoR1 and Pst1 instead of Xba1 and Spe.
* Repeated digest and ligation protocol from the weekend, with EcoR1 and Pst1 instead of Xba1 and Spe.


Revision as of 07:54, 5 August 2008

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Kdp?

  • There were transformants from yesterday's transformation
James celebrates apparent success
  • After Biobrick PCR the gel showed short fragments, suggesting self ligation of the vector.
Ellis realises the sad truth
  • Repeated digest and ligation protocol from the weekend, with EcoR1 and Pst1 instead of Xba1 and Spe.

Biobricks

  • Extracted two GFP-only sequences (J04630 and I714062) from the registry, for use as reporters for osmY.
  • PCR on these and the RBS B0030. Ran E-gel to check PCR.



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