IGEM:Harvard/2006/Cyanobacteria
From OpenWetWare
<html><style type='text/css'> .tabs {
font-size:80%;
font-weight:none;
width: 100%;
color: #FFFFFF;
background:#FFFFFF url("/images/5/54/DarkgreenTab-bg.gif") repeat-x bottom;
}
.tabs li {
background:url("/images/3/36/DarkgeenTab-left.gif") no-repeat left top;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;
color:#FFFFFF;
padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#CCFF00;
background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:#66FF00;
}
</style></html>
Construct Planning
Synthesis
Agenda
See image at right for our long-term project outline.
Click here for our current agenda
Kai Gene sizes
- KaiA size: 855bp
- 903bp with BioBrick ends
- KaiB: 309bp
- 357bp with BioBrick ends
- KaiC: 1560bp
- KaiA size: 855bp
BioBricks Used
- <bbpart>BBa_J04450</bbpart>
- RFP device
- Insert size: 1069bp
- [pSB1A2]
- High-copy, AmpR
- Size: 2079bp
- <bbpart>BBa_J04500</bbpart>
- Lac promoter + RBS
- Insert size: 220bp
- [pSB1AK3]
- High-copy, AmpR, KanR
- Insert size: 3189bp
- [pSB4A3]
- Low-copy, AmpR
- Insert size: 3339 bp
- <bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart>
- GFP device
- Insert size: 995 bp
- <bbpart>BBa_J04450</bbpart>
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
- Week 6 progress update, upd. 10:02 7/24 HH
- Week 7 progress update
- Week 8 progress update
- Week 9 progress update
- Week 10 progress update, 50% complete
- Final Presentation (incomplete)
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
List of abbreviations:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
4 May 2024
| 11:36 | User:Jernej Turnsek diffhist +50 Jernej Turnsek talk contribs | ||||
|
|
09:20 | User:Massih Forootan 3 changes history +83 [Massih Forootan (3×)] | |||
|
|
09:20 (cur | prev) +78 Massih Forootan talk contribs (→Link to Publications) | ||||
|
|
09:18 (cur | prev) 0 Massih Forootan talk contribs (→Skills) | ||||
|
|
09:18 (cur | prev) +5 Massih Forootan talk contribs (→Skills) | ||||
|
|
07:28 | CHEM-ENG590E:Wiki Textbook 4 changes history −445 [Sarah L. Perry (4×)] | |||
|
|
07:28 (cur | prev) +84 Sarah L. Perry talk contribs (→Chapter 4 - Flow Control and Mixing) | ||||
|
|
07:21 (cur | prev) −490 Sarah L. Perry talk contribs (→Chapter 9 - Microfluidics and Cell Culture) | ||||
|
|
07:19 (cur | prev) −35 Sarah L. Perry talk contribs (→Chapter 9 - Microfluidics and Cell Culture) | ||||
|
|
07:18 (cur | prev) −4 Sarah L. Perry talk contribs (→Chapter 4 - Flow Control and Mixing) | ||||
| 07:18 | Move log Sarah L. Perry talk contribs moved page Microfluidic Gradient Generators - Greg Schneider to Microfluidic Gradient Generators (Adding an Author) | ||||
| 00:15 | Renhao Li Lab:Publications diffhist +265 Renhao Li talk contribs (→2024) | ||||
3 May 2024
| 15:02 | CHIP:Talks diffhist 0 Gabor Balazsi talk contribs | ||||
| 15:01 | CHIP:Data diffhist −23 Gabor Balazsi talk contribs | ||||
|
|
14:33 | UA Biophysics:Protocols:Kanamycin 5 changes history −421 [Elizabeth Suesca (5×)] | |||
|
|
14:33 (cur | prev) +13 Elizabeth Suesca talk contribs | ||||
|
|
14:21 (cur | prev) +16 Elizabeth Suesca talk contribs (→Materials) | ||||
|
|
14:19 (cur | prev) −6 Elizabeth Suesca talk contribs | ||||
|
|
14:14 (cur | prev) −50 Elizabeth Suesca talk contribs | ||||
|
|
14:13 (cur | prev) −394 Elizabeth Suesca talk contribs | ||||
|
|
14:33 | UA Biophysics:Protocols:Ampicillin 4 changes history −379 [Elizabeth Suesca (4×)] | |||
|
|
14:33 (cur | prev) +15 Elizabeth Suesca talk contribs | ||||
|
|
14:19 (cur | prev) −7 Elizabeth Suesca talk contribs | ||||
|
|
14:11 (cur | prev) 0 Elizabeth Suesca talk contribs (→PROTOCOL) | ||||
|
|
14:09 (cur | prev) −387 Elizabeth Suesca talk contribs | ||||
|
|
14:31 | UA Biophysics:Protocols:Erythromycin 3 changes history −315 [Elizabeth Suesca (3×)] | |||
|
|
14:31 (cur | prev) −7 Elizabeth Suesca talk contribs | ||||
|
|
14:30 (cur | prev) −12 Elizabeth Suesca talk contribs (→Materials) | ||||
|
|
14:18 (cur | prev) −296 Elizabeth Suesca talk contribs | ||||
|
|
14:29 | UA Biophysics:Protocols:Chloramphenicol 2 changes history −427 [Elizabeth Suesca (2×)] | |||
|
|
14:29 (cur | prev) +36 Elizabeth Suesca talk contribs | ||||
|
|
14:16 (cur | prev) −463 Elizabeth Suesca talk contribs | ||||
|
|
N 12:09 | BioMicroCenter:Oligo Synthesis 7 changes history +4,408 [Noelani Kamelamela (7×)] | |||
|
|
12:09 (cur | prev) −14 Noelani Kamelamela talk contribs (→Dr Oligo 96) | ||||
|
|
12:09 (cur | prev) −16 Noelani Kamelamela talk contribs (→STX-200) | ||||
|
|
12:08 (cur | prev) −46 Noelani Kamelamela talk contribs (→Dr Oligo 96) | ||||
|
|
12:08 (cur | prev) +35 Noelani Kamelamela talk contribs (→Dr Oligo 96) | ||||
|
|
08:29 (cur | prev) +7 Noelani Kamelamela talk contribs (→Dr Oligo 96) | ||||
|
|
08:27 (cur | prev) +59 Noelani Kamelamela talk contribs (→Dr Oligo 96) | ||||
| N |
|
08:16 (cur | prev) +4,383 Noelani Kamelamela talk contribs (Created page with "{{BioMicroCenter}} ''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR> The BioMicro Center currently hosts two oligo synthesizers: one Dr Oligo 96 (Biolytic) and one Syntax STX-200 (DNAScript). <br><br> Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the...") | |||
|
|
12:06 | BioMicroCenter:Covaris 5 changes history +172 [Noelani Kamelamela (5×)] | |||
|
|
12:06 (cur | prev) −5 Noelani Kamelamela talk contribs (→R230) | ||||
|
|
09:55 (cur | prev) +2 Noelani Kamelamela talk contribs (→R230) | ||||
|
|
09:54 (cur | prev) +1 Noelani Kamelamela talk contribs (→R230) | ||||
|
|
09:52 (cur | prev) +4 Noelani Kamelamela talk contribs (→R230) | ||||
|
|
08:41 (cur | prev) +170 Noelani Kamelamela talk contribs (→R230) | ||||
|
|
09:52 | (Upload log) [Noelani Kamelamela (6×)] | |||
|
|
09:52 Noelani Kamelamela talk contribs uploaded File:R230pic.jpg | ||||
|
|
08:42 Noelani Kamelamela talk contribs uploaded File:R230pic.jpeg | ||||
|
|
08:32 Noelani Kamelamela talk contribs uploaded File:Syntax.jpg | ||||
|
|
08:31 Noelani Kamelamela talk contribs uploaded File:Droligoprocess.jpg | ||||
|
|
08:29 Noelani Kamelamela talk contribs uploaded File:Chamber.jpg | ||||
|
|
08:28 Noelani Kamelamela talk contribs uploaded File:DrOligo.jpg | ||||
2 May 2024
|
|
19:54 | Paper Microfluidic Device for Archiving Breast Epithelial Cells 6 changes history −389 [Xning098 (6×)] | |||
|
|
19:54 (cur | prev) −10 Xning098 talk contribs (→Diseases tested with neonatal heel pricks) | ||||
|
|
19:53 (cur | prev) −12 Xning098 talk contribs (→Results with Breast Epithelial Cells) | ||||
|
|
19:49 (cur | prev) 0 Xning098 talk contribs (→Current diagnostic tools) | ||||
|
|
19:45 (cur | prev) +45 Xning098 talk contribs (→Design Parameters) | ||||
|
|
19:40 (cur | prev) −412 Xning098 talk contribs (→Design Parameters) | ||||
|
|
19:35 (cur | prev) 0 Xning098 talk contribs (→Paper Microfluidics) | ||||
