IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/15: Difference between revisions
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== | ==Team Flavour== | ||
=Primer Designs for Agrobacterium Vector= | |||
*Primers were designed to amplify from the Expression Series pORE Agrobacterium plasmid (e3) 1)the pENTcup2 promoter, 2) the tNOS stop sequence, 3) the tNOS stop sequence + additional stop codon and 4) the pHLP promoter. | *Primers were designed to amplify from the Expression Series pORE Agrobacterium plasmid (e3) 1)the pENTcup2 promoter, 2) the tNOS stop sequence, 3) the tNOS stop sequence + additional stop codon and 4) the pHLP promoter. | ||
pENTcup2_BB_F | pENTcup2_BB_F | ||
Revision as of 07:35, 15 June 2010
 iGEM Project name 1
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Team FlavourPrimer Designs for Agrobacterium Vector
pENTcup2_BB_F CCTTTCTAGAGGGATCTTCTGCAAGCATCT pENTcup2_BB_R AAGGCTGCAGCGGCCGCTACTAGTTCCGGTGGGTTTTGAGGT STOP_NOSterm_BB_F CCTTTCTAGATGAGATCGTTCAAACATTTGG NOSterm_BB_F CCTTTCTAGAGATCGTTCAAACATTTGGCA NOSterm_BB_R AAGGCTGCAGCGGCCGCTACTAGTGATCTAGTAACATAGATGACA pHPL_BB_F CCTTTCTAGAAACGTGGATACTTGGCAGTG pHPL_BB_R AAGGCTGCAGCGGCCGCTACTAGTCTTTTGAGCTTAGAGGTTTTT
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