IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/06/15: Difference between revisions

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Banana Pathway:
Banana Pathway:
BioBrick Part #: BBa_J45014 <br>
[[Image:codon_table_BBa_J45014.jpg|150px|BBa_J45014]]
[[Image:codon_table_BBa_J45014_pg_2.jpg|150px|BBa_J45014]]
[[Image:Codon_table_BBa_J45014_pg_3.jpg|150px|BBa_J45014]]


[[Image:codon_table_BBa_J45014.jpg|150px|abc]]
BioBrick Part #: BBa_J45250
[[Image:codon_table_BBa_J45014_pg_2.jpg|150px|abc]]
[[Image:codon_table_BBa_J45250_pg1.jpg|150px|BBa_J45250]]
[[Image:Codon_table_BBa_J45014_pg_3.jpg|150px|abc]]
[[Image:codon_table_BBa_J45250_pg2.jpg|150px|BBa_J45250]]
 
[[Image:codon_table_BBa_J45250_pg3.jpg|150px|BBa_J45250]]
[[Image:codon_table_BBa_J45250_pg1.jpg|150px|abc]]
[[Image:codon_table_BBa_J45250_pg4.jpg|150px|BBa_J45250]]
[[Image:codon_table_BBa_J45250_pg2.jpg|150px|abc]]
[[Image:codon_table_BBa_J45250_pg3.jpg|150px|abc]]
[[Image:codon_table_BBa_J45250_pg4.jpg|150px|abc]]


Valencene Pathway:
Valencene Pathway:

Revision as of 09:03, 15 June 2010

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Team Flavour

Primer Designs for Agrobacterium Vector

  • Primers were designed to amplify from the Expression Series pORE Agrobacterium plasmid (e3) 1)the pENTcup2 promoter, 2) the tNOS stop sequence, 3) the tNOS stop sequence + additional stop codon and 4) the pHLP promoter.

Note: the NOSterm_BB_R primer works for both tNOS and tNOS+STOP codon sequences. 

   pENTcup2_BB_F
   CCTTTCTAGAGGGATCTTCTGCAAGCATCT

   pENTcup2_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTTCCGGTGGGTTTTGAGGT

   STOP_NOSterm_BB_F
   CCTTTCTAGATGAGATCGTTCAAACATTTGG

   NOSterm_BB_F
   CCTTTCTAGAGATCGTTCAAACATTTGGCA

   NOSterm_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTGATCTAGTAACATAGATGACA

   pHPL_BB_F
   CCTTTCTAGAAACGTGGATACTTGGCAGTG

   pHPL_BB_R
   AAGGCTGCAGCGGCCGCTACTAGTCTTTTGAGCTTAGAGGTTTTT

BioBrick Transformation

Colonies were observed after overnight culture growth, but in low quantities.

Codon Usage in Arabidopsis

Wintergreen Pathway:
BioBrick Part #: BBa_J45700

BBa_J45700 BBa_J45700 BBa_J45700 BBa_J45700

Banana Pathway: BioBrick Part #: BBa_J45014
BBa_J45014 BBa_J45014 BBa_J45014

BioBrick Part #: BBa_J45250 BBa_J45250 BBa_J45250 BBa_J45250 BBa_J45250

Valencene Pathway:

Valencene Valencene Valencene




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