IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Design: Difference between revisions

Revision as of 16:04, 29 October 2006

Super Parts	Molecular Prey-Predator Oscillator
Actual Part	Logo of the Part
Sub Parts	Test Sensing Prey Construct	Test Prey Construct

Design allows for an exponential increase of AHL via a positive feedback loop
- Interaction between LuxR, AHL and promoter controlling expression of LuxI
Design allows for assesment of status of positive feedback loop via:
- GFP Production - Linked to LuxI expression
- AHL Production - Can be indirectly assessed via T9002 assay

INPUTS	biological component	comments
AHL	LuxR + pLuxR	These components sense input and activate output
OUTPUTS	biological component	comments
AHL	LuxI	LuxI expression causes enzymatic generation of AHL

@@ Line 23: / Line 23: @@
 <br style="clear:both">
-*Design
+==Registry==
-*Desing
-*Design
+===J37015===
+[[Image:J37015.JPG]]
+[http://parts.mit.edu/registry/index.php/Part:BBa_J37015 J37015 @ MIT Parts Registry]
+==Design Choices==
+*Design allows for an exponential increase of AHL via a positive feedback loop
+**Interaction between LuxR, AHL and promoter controlling expression of LuxI
+*Design allows for assesment of status of positive feedback loop via:
+**GFP Production - Linked to LuxI expression
+**AHL Production - Can be indirectly assessed via T9002 assay
+{| border="1" width="100%"
+|- style="background:lightgreen"
+! INPUTS !! biological component !! comments
+|-
+|AHL||LuxR + pLuxR||These components sense input and activate output
+|- style="background:orange"
+! OUTPUTS !! biological component !! comments
+|-
+|AHL||LuxI||LuxI expression causes enzymatic generation of AHL
+|}