IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation: Difference between revisions

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!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
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|-
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || [http://openwetware.org/images/9/9f/Sequence_alignment_22nd_sep.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
|-
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || [http://openwetware.org/images/b/b1/J37015_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|}
|}



Revision as of 07:42, 30 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
Yes Yes Primers used
<bbpart>J37015</bbpart>
Yes Yes Primers used

WetLab work entries

  • Part has been successfully built.
  • Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.