IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Prey Construct/Implementation

From OpenWetWare

< IGEM:IMPERIAL | 2006 | project | Oscillator | project browser | Test Prey Construct(Difference between revisions)
Jump to: navigation, search
Current revision (19:46, 29 October 2006) (view source)
(Ligation Strategy)
 
Line 26: Line 26:
==Ligation Strategy==
==Ligation Strategy==
-
*<bbpart>F2620</bbpart> (6B) + <bbpart>J37034</bbpart>
+
<u><font size="2">'''Ligating the Prey Construct:'''</font></u>
-
**J37024 (insert), cut with XbaI & PstI, expected size = 1544 bp
+
__NOTOC__
-
**F2620 (6B) (vector), cut with SpeI & PstI, expected size = 1061 bp + 2079 bp (pSB1A2) = 3140 bp
+
{| border="1" cellpadding="0" width="100%"
-
*Miniprep sizes expected: 2613 bp & 2079 bp (pSB1A2)
+
|-
-
*Status: Completed & Sequenced
+
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2
 +
|-
 +
!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center>
 +
|-
 +
!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center>
 +
|}
 +
 
 +
 
 +
==Quality control==
 +
__NOTOC__
 +
{| border="1" cellpadding="0" width="100%"
 +
|-
 +
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing
 +
|-
 +
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|-
 +
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
 +
|}
 +
 
 +
==WetLab work entries==
 +
 
 +
*Prey has been successfully ligated and needs to undergo data analysis and parameter extraction
 +
*Methods for controlling the positive feedback loop (Dilution method, Riboswitch & PoPs blocker) have to be incorporated and tested.

Current revision

Super Parts Prey Construct
Actual Part
Sub Parts F2620 C0261 I13401


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
Image:C0261 logo.pngImage:I13504 logo.png
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)
Image:J37015 logo.png


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
Image:J37034 logo.png Yes ? ?
<bbpart>J37015</bbpart>
Image:J37015 logo.png Yes ? ?

WetLab work entries

  • Prey has been successfully ligated and needs to undergo data analysis and parameter extraction
  • Methods for controlling the positive feedback loop (Dilution method, Riboswitch & PoPs blocker) have to be incorporated and tested.
Personal tools