IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation: Difference between revisions
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*[[Knight:Electroporation]] | *[[Knight:Electroporation]] | ||
*[[Endy:High-efficiency electroporation]] | *[[Endy:High-efficiency electroporation]] | ||
*Incubation | |||
1. Degradation time | 1. Degradation time |
Revision as of 07:39, 27 July 2007
Cell by Date: Implementation
List of Required Protocols
Preliminary Preparation
Phase 1
Preliminery Data
Protocols Required:
1. Degradation time
- Endy:RNA Half-life
- λ-Reporters
2. Rate of expression at constant temperature
- Fluorometer Reading
- Noting down colour change (photographs)
3. Range of Reporters
- Flurometer Reading
- RFP
- DsRed
- Beta-Galactosidase_Assay_(A_better_Miller)
- β-galactoside - requires subtrates X-GAL
- Luciferase - requires substrate ATP, O2, and luciferin
4. Range of promoters
Refer to list of Required Parts
Data Analysis
Refer to modelling section
Validation of Model
1. Validate Vmax value
2. Validate transient response using best promoters/reporters
3.