IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation: Difference between revisions

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=====Preliminery Data=====
====Preliminery Data====


Protocols Required:
Protocols Required:
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*Incubation
*Incubation


1. Degradation time
=====1. Degradation time=====
*[[Endy:RNA Half-life]]
*[[Endy:RNA Half-life]]
*λ-Reporters
*λ-Reporters


2. Rate of expression at constant temperature
 
=====2. Rate of expression at constant temperature=====
*Fluorometer Reading
*Fluorometer Reading
*Noting down colour change (photographs)
*Noting down colour change (photographs)


3. Range of Reporters
 
=====3. Range of Reporters=====
*Flurometer Reading
*Flurometer Reading
**RFP
**RFP
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*Luciferase - requires substrate ATP, O<sub>2</sub>, and luciferin
*Luciferase - requires substrate ATP, O<sub>2</sub>, and luciferin


4. Range of promoters
 
=====4. Range of promoters=====


Refer to list of Required Parts
Refer to list of Required Parts


=====Data Analysis=====
====Data Analysis====


Refer to modelling section
Refer to modelling section




=====Validation of Model=====
====Validation of Model====


1. Validate V<sub>max</sub> value
=====1. Validate V<sub>max</sub> value=====
*Fluorometer Reading
*Noting down colour change (photographs)
 
 
=====2. Validate transient response using best promoters/reporters=====
*Fluorometer Reading
*Noting down colour change (photographs)


2. Validate transient response using best promoters/reporters


3.


===Phase 2===
===Phase 2===

Revision as of 07:41, 27 July 2007

Cell by Date: Implementation



List of Required Protocols

Preliminary Preparation


Phase 1

Preliminery Data

Protocols Required:

1. Degradation time


2. Rate of expression at constant temperature
  • Fluorometer Reading
  • Noting down colour change (photographs)


3. Range of Reporters
  • Flurometer Reading
    • RFP
    • DsRed
  • Luciferase - requires substrate ATP, O2, and luciferin


4. Range of promoters

Refer to list of Required Parts

Data Analysis

Refer to modelling section


Validation of Model

1. Validate Vmax value
  • Fluorometer Reading
  • Noting down colour change (photographs)


2. Validate transient response using best promoters/reporters
  • Fluorometer Reading
  • Noting down colour change (photographs)


Phase 2

Phase 3

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