IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/30: Difference between revisions

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(Autocreate 2013/08/30 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 08/30/13</b> <b>People in lab: Kelsey Crossen</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/28 PCR products


<b>Start Time:</b>
<b>Start Time:</b> 9:00 AM


<b>Purpose:</b>  
<b>Purpose:</b> Check if PCR was successful


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> iGEM LTM ed. 2
 
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| Entry || Entry || Entry || #
|-
| Entry || Entry || Entry || #
|}


{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
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! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
|-
| Sample || || || ||  ||  ||  ||  ||  
| Sample || Ladder || norCB A 8/28 || nosZ A 8/28 ||  ||  ||  ||  ||  
|}  
|}  


<b>Results:</b>  
<b>Results:</b> No product visible
 
<b>Stop Time:</b>  


<b>Notes:</b>  
<b>Next:</b> Adjust PCR and try again


<b>Stop Time:</b>  
== <b>Date: 08/30/13</b> <b>People in lab: David Pohlman</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> Making competent cell seed plates
 
<b>Purpose:</b> To grow fresh DH5alpha cells for making competent cells


<b>Next:</b>
<b>Protocol:</b> iGEM LTM ed. 2 - Streaking from frozen stock


<b>Products:</b> 2 Seed DH5alpha 8/30/13


<b>Next:</b> Inoculate into LB broth


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Revision as of 21:43, 16 May 2014

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Date: 08/30/13 People in lab: Kelsey Crossen

Title: Gel electrophoresis of 8/28 PCR products

Start Time: 9:00 AM

Purpose: Check if PCR was successful

Protocol: iGEM LTM ed. 2

Well 1 2 3 4 5 6 7 8
Sample Ladder norCB A 8/28 nosZ A 8/28

Results: No product visible

Stop Time:

Next: Adjust PCR and try again

Date: 08/30/13 People in lab: David Pohlman

Title: Making competent cell seed plates

Purpose: To grow fresh DH5alpha cells for making competent cells

Protocol: iGEM LTM ed. 2 - Streaking from frozen stock

Products: 2 Seed DH5alpha 8/30/13

Next: Inoculate into LB broth


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