IGEM:UNAM LCG/2009/Notebook/Hydrobium etli/2011/09/29: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Reactive''' | | align="center" style="background:#f0f0f0;"|'''Reactive''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Amount (μL)''' | ||
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Revision as of 16:30, 29 September 2011
Hydrobium etli | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||||||
pBBR1MCS-5 Double digestionAbstractI made a double digestion with PstI and EcoRI of the plasmid pBBR1MCS-5 that Paulina extracted. I need this plasmid digested to ligate all the anderson collection promoters. DetailsI do not exctly know the plasmid pBBR1MCS-5 concentration or even if the extraction was good, but Paulina says it was, I have to ask her for the gel image. I made 4 reactions, 3 were from 3 different pBBR1MCS-5 extractions or samples and one was as a control from a part that Fabricio had already made and went well, this part has a RFP. Reactive amounts and details
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