IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19: Difference between revisions
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1. PCR - AID for translation fusion | 1. PCR - AID for translation fusion | ||
Primers: | |||
AIDlNrH | AIDlNrH<br> | ||
AIDpLinB | AIDpLinB | ||
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2. PCR - T7 RNA-polimerase for translation fusion | 2. PCR - T7 RNA-polimerase for translation fusion | ||
Primers: | |||
T7lLinkB | T7lLinkB<br> | ||
T7pXbSal | T7pXbSal | ||
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3. PCR - T7 RNA-polimerase for transcription fusion | 3. PCR - T7 RNA-polimerase for transcription fusion | ||
Primers: | |||
T7lRBSHi | T7lRBSHi<br> | ||
T7pXbSal | T7pXbSal | ||
Revision as of 02:37, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. PCR - AID for translation fusion Primers:
AIDlNrH Template DNA: pTrc99A-AID Annealing temperature: 55 °C Annealing time: 60 sec 20 cycles
Primers:
T7lLinkB Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
Primers:
T7lRBSHi Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles | |
