IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19: Difference between revisions
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1. PCR | 1. PCR - AID for translation fusion | ||
Primers: | |||
AIDlNrH | |||
AIDlNrH<br> | |||
AIDpLinB | AIDpLinB | ||
Template DNA: | |||
pTrc99A-AID | pTrc99A-AID | ||
| Line 23: | Line 24: | ||
20 cycles | 20 cycles | ||
2. PCR - T7 RNA-polimerase for translation fusion | |||
T7lLinkB | |||
Primers: | |||
T7lLinkB<br> | |||
T7pXbSal | T7pXbSal | ||
Template DNA: | |||
E. coli Rosetta genomic DNA | E. coli Rosetta genomic DNA | ||
| Line 40: | Line 43: | ||
20 cycles | 20 cycles | ||
3. PCR - T7 RNA-polimerase for transcription fusion | |||
T7lRBSHi | |||
Primers: | |||
T7lRBSHi<br> | |||
T7pXbSal | T7pXbSal | ||
Template DNA: | |||
E. coli Rosetta genomic DNA | E. coli Rosetta genomic DNA | ||
Revision as of 02:38, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. PCR - AID for translation fusion Primers: AIDlNrH Template DNA: pTrc99A-AID Annealing temperature: 55 °C Annealing time: 60 sec 20 cycles
Primers: T7lLinkB Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
Primers: T7lRBSHi Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles | |
