IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19: Difference between revisions
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AIDpLinB | AIDpLinB | ||
Template DNA: | |||
pTrc99A-AID | pTrc99A-AID | ||
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T7pXbSal | T7pXbSal | ||
Template DNA: | |||
E. coli Rosetta genomic DNA | E. coli Rosetta genomic DNA | ||
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T7pXbSal | T7pXbSal | ||
Template DNA: | |||
E. coli Rosetta genomic DNA | E. coli Rosetta genomic DNA | ||
Revision as of 01:21, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. PCR - AID for translation fusion Starters: AIDlNrH AIDpLinB Template DNA: pTrc99A-AID Annealing temperature: 55 °C Annealing time: 60 sec 20 cycles
Starters: T7lLinkB T7pXbSal Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
Starters: T7lRBSHi T7pXbSal Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles | |
