Klapperich Lab:Notebook/Lab Meeting Notes/2008/10/07: Difference between revisions

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†RNA project
†RNA project
* Sonali will teach Stephanie Jurkat Culture. <br>
* Jeff Braman will visit in early Dec. <br>
* Jeff Braman will visit in early Dec. <br>
* We have Anirban's protocol and system etc. Issues resolved.<br>
* SM and AC will do first experiment on Friday. Update? <br>
* Stephanie working with Paul to make more straws. <br>
* SM and AC will do first experiment on Friday. <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark finish control circuit (Temp, humidity, pressure) design and ordered parts <br>  
* Mark finish control circuit (Temp, humidity, pressure) design and ordered parts <br>  
* Fluorescence microscopy available from 720. Try beads experiment. <br>
* Fluorescence microscopy available from 720. Try beads experiment. Update? <br>
* Cutter plotter ordered to make new fluidic layer <br>
* Cutter plotter arrived.<br>
* Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.  <br>
* Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.  <br>
* Discuss dispensing from evap chip in a real world way. <br>
* Discuss dispensing from evap chip in a real world way. <br>
* Current design gives E.coli signal, clotting is still a problem <br>
* Current design gives E.coli signal, clotting is still a problem <br>
* Change of design: funnel-shaped wells, smooth hole, make samples in pipette tips? <br>
* Change of design: funnel-shaped wells, smooth hole, make samples in pipette tips? <br>
* Jane working on CIMIT fellowship presentation and future plans for THESIS! <br>
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<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>

Revision as of 09:28, 7 October 2008

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7 October 2008 Lab Meeting Agenda

† Sonali's research report today.

† Announcements

  • A slow migration to 709 can now begin.


† Flu R01

  • More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? YES. Cathie will amend.
  • Plaque assay status.
  • Cathie STILL STILL has to submit the progress report to INSPIR.


† SEPSIS Project - CIMIT Bird Flu

  • Sample (raw pellets and film) from Ticona COC ordered.
  • B sub in blood is done. Lets talk about comepleting the gram positive plots so they "match."
  • SEM image analysis of pore size. Progress?


†RNA project

  • Jeff Braman will visit in early Dec.
  • SM and AC will do first experiment on Friday. Update?

† COBRA

  • Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
  • Fluorescence microscopy available from 720. Try beads experiment. Update?
  • Cutter plotter arrived.
  • Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.
  • Discuss dispensing from evap chip in a real world way.
  • Current design gives E.coli signal, clotting is still a problem
  • Change of design: funnel-shaped wells, smooth hole, make samples in pipette tips?


† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa. Hopefully this week.
  • Update: Monday 29 Sept Frank contacted Shane Conran of Superior Fluid Solutions (referred by Jess from UCSB). Will draw up preliminary design and send to us for evaluation.
  • Update: Monday 6 Oct Haven't heard anything from Shane yet. Attempting to contact again, so far without success.


† Fraunhofer: LOAC

  • Alex working with Paul. (something called personal lubricant)
  • Alex working on more toluene bonding issues.
  • Try Ticona material. (HM, JD, AG)
  • Still Still Still troubleshooting ??


† Biointerfaces group

  • Make a mold for Brett this week. When mask is made.
  • Do preliminary E. coli experiment soon.
  • Making a CR mask Tuesday afternoon.


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • PCR areas defined. Protocols reviewed. Contamination issues discussed....
  • QQ tested Taqman assays. Two probe sets. One set is better than the other.
  • Contamination. Make an internal control on the chip.
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed (JD)
  • Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.
  • MinCheol is making Four simple PCR models to optimize temperature distribution along to the channel. Will do another model with the insulation on the bottom.
  • MinCheol is simulating DNA's Residence Time for simple PCR models. The DNA particles need to be much smaller. Also, consult the Lab on Chip paper that JD mentioned.


† RCA

  • Cathie Still Working on Whitepaper almost done!
  • Send PAs to Sonali and Jaephil for sending RCA grant on to someplace else.


† Senior project

  • Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources.
  • Haven't seen Teddy yet.



† F31: Cochlea

  • ARO Abstract due October 1st.
  • Data summary focusing on the fundamental biological questions for next week.


† Silica Optimization (Lambda):

  • Ongoing. More concentrations. Forged chips.
  • Pressure testing on these channels when they are flowing.


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