Kubke Lab:/Notebook/Cranial nerve development/2010/12/02: Difference between revisions
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{{Note|Please clarify the type of glass slides you had been mounting your sections on, and also, what embryos were stained here?--[[User:M Fabiana Kubke|MF Kubke]] 04:28, 20 January 2011 (EST)}} | {{Note|Please clarify the type of glass slides you had been mounting your sections on, and also, what embryos were stained here?--[[User:M Fabiana Kubke|MF Kubke]] 04:28, 20 January 2011 (EST)}} | ||
**The slides used were clean, unsubbed [http://www.menzel.de/Microscope_Slides.687.0.html?L=1 Original Menzel-Glaser] Cut Edges, Frosted Ends. --[[User:Reuben Cutfield|Reuben Cutfield]] 18:51, 20 January 2011 (EST) | **The slides used were clean, unsubbed [http://www.menzel.de/Microscope_Slides.687.0.html?L=1 Original Menzel-Glaser] Cut Edges, Frosted Ends. --[[User:Reuben Cutfield|Reuben Cutfield]] 18:51, 20 January 2011 (EST) | ||
{{Note|the information here does not match that on the embryo sheet. The sheet says that the sections were not used for staining and there is no mention of the sections falling off the slides. You also need to specify the slides were not subbed. Please go through the information carefully and rectify your records--[[User:M Fabiana Kubke|MF Kubke]] 04:18, 3 February 2011 (EST)}} | |||
Decided we definitely need subbed slides. We're going to try using polylysine subbed slides. | Decided we definitely need subbed slides. We're going to try using polylysine subbed slides. |
Revision as of 02:18, 3 February 2011
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General Entries
Decided we definitely need subbed slides. We're going to try using polylysine subbed slides.
SECTIONS CURLING EXCESSIVELY ON PLATFORM AND PINCHING AT EDGE OF GLASS Personal EntriesReuben
MalishaRESULTS STILL POOR. OCT was rubbery therefore we decided to start with a new embryo--Malisha Hettiarachchi 21:25, 12 December 2010 (EST). Staged an embryo labelled "stage 28" to confirm and labelled as MH002--Malisha Hettiarachchi 21:25, 12 December 2010 (EST)
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