Magni:Protocols/LB
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LB medium (liquid)
- For every 1 L of medium, add to a pyrex bottle or flask:
- 10 g NaCl
- 10 g bacto-tryptone
- 5 g yeast extract
- Add 1 L of deionized water (blue tap of the Millipore machine).
- Autoclave (if using a bottle, do not close the cap completely).
- Let it cool to about 40degC and, when required, add antibiotic near the flame.
- Store in the fridge and always check for contaminations before using.