Smolke:Protocols
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Current revision (15:30, 16 October 2012) (view source) (→Yeast) |
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:*[[Smolke:Protocols/Gel extraction|Gel extraction]] | :*[[Smolke:Protocols/Gel extraction|Gel extraction]] | ||
:*[[Smolke:Protocols/Ligation|Ligation]] | :*[[Smolke:Protocols/Ligation|Ligation]] | ||
| - | :*[[Smolke:Protocols/Transformation|Transformation]] | + | :*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]] |
| + | :*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]] | ||
:*[[Smolke:Protocols/Screening|Colony PCR]] | :*[[Smolke:Protocols/Screening|Colony PCR]] | ||
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]] | :*[[Smolke:Protocols/Digest Screening|Screening by digestion]] | ||
| Line 19: | Line 20: | ||
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]] | :*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]] | ||
:*[[Smolke:Protocols/Gene assembly|Gene assembly]] | :*[[Smolke:Protocols/Gene assembly|Gene assembly]] | ||
| + | :*[[Smolke:Protocols/EtBr stock|EtBr stock]] | ||
===Yeast=== | ===Yeast=== | ||
| Line 25: | Line 27: | ||
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]] | :*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]] | ||
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]] | :*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]] | ||
| - | :*[[Smolke:Protocols/ | + | :*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]] |
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]] | :*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]] | ||
:*[[Smolke:Protocols/Western|Anti-V5 Western]] | :*[[Smolke:Protocols/Western|Anti-V5 Western]] | ||
| - | :*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations]] | + | :*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]] |
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]] | :*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]] | ||
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]] | :*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]] | ||
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:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]] | :*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]] | ||
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]] | :*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]] | ||
| + | |||
| + | ===VYB=== | ||
| + | :*[[Smolke:Protocols/Guidelines|Guidelines]] | ||
===Miscellaneous=== | ===Miscellaneous=== | ||
Current revision
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