Smolke:Protocols

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(Cloning)
(Yeast)
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:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Ligation|Ligation]]
:*[[Smolke:Protocols/Ligation|Ligation]]
-
:*[[Smolke:Protocols/Electrocompetent Transformation|Transformation]]
+
:*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]]
 +
:*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
Line 19: Line 20:
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
 +
:*[[Smolke:Protocols/EtBr stock|EtBr stock]]
===Yeast===
===Yeast===
Line 28: Line 30:
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
-
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations]]
+
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]]
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
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:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
 +
 +
===VYB===
 +
:*[[Smolke:Protocols/Guidelines|Guidelines]]
===Miscellaneous===
===Miscellaneous===

Revision as of 14:30, 16 October 2012

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Note: If possible, please follow the standard protocol format.

Contents

Protocols

Cloning

Yeast

Protocols
Observations and Discussion

Tissue Culture room

Quanta

VYB

Miscellaneous

Solutions

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