User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/11/06: Difference between revisions

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==Procedure==
==Procedure==
===Stock Solution Prep===
===Stock Solution Prep===
*Gold- .011 grams was added to a 10 ml volumetric flasks and was filled to 10 ml with water.  
*Gold- .011 grams was added to a 10 ml volumetric flask and was filled to 10 ml with water.  
*Hemoglobin- .0032 grams of hemoglobin was added to a 10 ml volumetric flask and was filled to 10 ml with water.
*Hemoglobin- .0032 grams of hemoglobin was added to a 10 ml volumetric flask and was filled to 10 ml with water.
*Lysozyme- .02 grams of lysozyme was added to a 25 ml volumetric flask and was filled to 25 ml with water.  
*Lysozyme- .02 grams of lysozyme was added to a 25 ml volumetric flask and was filled to 25 ml with water.  
**Specific concentrations are expressed below.
**Specific concentrations are expressed below.
 
===Sample Prep===
===Sample Prep===
*AuNP's were made as dictated below.
*AuNP's were made as dictated below.

Revision as of 11:07, 6 November 2013

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Objective

  • Synthesize more Lysozyme linked AuNP's and Hemoglobin lined AuNP's.

Procedure

Stock Solution Prep

  • Gold- .011 grams was added to a 10 ml volumetric flask and was filled to 10 ml with water.
  • Hemoglobin- .0032 grams of hemoglobin was added to a 10 ml volumetric flask and was filled to 10 ml with water.
  • Lysozyme- .02 grams of lysozyme was added to a 25 ml volumetric flask and was filled to 25 ml with water.
    • Specific concentrations are expressed below.

Sample Prep

  • AuNP's were made as dictated below.

  • Once the solutions were mixed they were heated for 4 hours at 80 degrees Celsius.

Data


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