User:Anthony Salvagno/Notebook/Research/2011/03/04/Prepping Unzipping With pALS: Difference between revisions
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(New page: {{qn}} ==PCR== I cleaned up the PCR reaction from Thursday and the nanodrop revealed: *227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA) ==BstXI Digestion== {{ShowGoogleExcel|id=0Agbdciapt...) |
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*227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA) | *227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA) | ||
==BstXI Digestion== | ==BstXI Digestion== | ||
{{ShowGoogleExcel|id= | {{ShowGoogleExcel|id=0Agbdciapt4QZdFRQdlV3cGV5eHgzTEE2VnZGRGlua3c|width=500|height=300}} | ||
==Adapter Annealing== | ==Adapter Annealing== | ||
==Ligation== | ==Ligation== | ||
Revision as of 09:01, 4 March 2011
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PCR
I cleaned up the PCR reaction from Thursday and the nanodrop revealed:
- 227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA)
BstXI Digestion
{{#widget:Google Spreadsheet |key=0Agbdciapt4QZdFRQdlV3cGV5eHgzTEE2VnZGRGlua3c |width=500 |height=300 }}
