User:Anthony Salvagno/Notebook/Research/2011/03/04/Prepping Unzipping With pALS: Difference between revisions

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m (Quick-adding category Ant PCR (using HotCat))
m (Removed category Ant PCR; Quick-adding category Ant Digestion (using HotCat))
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There is a good chance this doesn't happen today.
There is a good chance this doesn't happen today.


[[Category:Ant PCR]]
 
[[Category:Ant Digestion]]

Revision as of 11:18, 4 March 2011

PCR

I cleaned up the PCR reaction from Thursday and the nanodrop revealed:

  • 227ng/ul --> 85nM in 30ul (total of 6.8ug of DNA)

BstXI Digestion

{{#widget:Google Spreadsheet |key=0Agbdciapt4QZdFRQdlV3cGV5eHgzTEE2VnZGRGlua3c |width=500 |height=300 }}

Adapter Annealing

Since I got a new bottom oligo with 5' biotinylation I will have to anneal a new adapter duplex. {{#widget:Google Spreadsheet |key=0Agbdciapt4QZdGVoNElGZ0piZ0FzR0hGOFdBWkhZdGc |width=500 |height=300 }}

Ligation

I only have SappBR so I will need to ligate the adapter to here first and then ligate this to the digested pALS.

SappBR-Adapter Ligation

{{#widget:Google Spreadsheet |key=0Agbdciapt4QZdHZpSjF5UThUek5JN3NtR1RtUkFoenc |width=500 |height=300 }}

ApBR-DpALS Ligation

There is a good chance this doesn't happen today.

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