User:Anugraha Raman/Notebook/iGEM 2010/2010/06/23: Difference between revisions

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[[Image: AmiRNA_pcr_scheme.jpg|thumb|center|amiRNA pcr diagram]]
[[Image: AmiRNA_pcr_scheme.jpg|thumb|center|amiRNA pcr diagram]]
**Go to the [[http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| amiRNA section]] for our primers for this multi step PCR
**Go to the [[http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| amiRNA section]] for our primers for this multi step PCR
'''Transformations'''
*Transformed ligations (V0210+LTP1) (V0210+Ger 3 sense) (V0210+Ger 3 antisense)w/turbo cells
*Grew up colonies w/ RS300 for minipreps


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Revision as of 11:48, 23 June 2010

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Wednesday June 23, 2010

amiRNA

amiRNA pcr diagram

Transformations

  • Transformed ligations (V0210+LTP1) (V0210+Ger 3 sense) (V0210+Ger 3 antisense)w/turbo cells
  • Grew up colonies w/ RS300 for minipreps