User:Anugraha Raman/Notebook/iGEM 2010/2010/06/23: Difference between revisions

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**Go to the [[http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| amiRNA section]] for our primers for this multi step PCR or see chart below:
**Go to the [[http://openwetware.org/wiki/IGEM:Harvard/2010/Team_Allergy| amiRNA section]] for our primers for this multi step PCR or see chart below:


{| class="wikitable" border ="1px solid black"
 
{| class="wikitable" border ="3px solid black"
|-
|-
|description
|'''Description'''
|forward (A)
|'''Forward (A)'''
|reverse (B)
|'''Reverse (B)'''
|-
|-
|biobrick RS300
|'''biobrick RS300'''
|''CCTTGAATTCGCGGCCGCATCTAGA'' CCCACAAACACACGCTCGGA
|''CCTTGAATTCGCGGCCGCATCTAGA'' CCCACAAACACACGCTCGGA
|''AAGGCTGCAGCGGCCGCTACTAGT'' CCCCATGGCGATGCCTTAAA
|''AAGGCTGCAGCGGCCGCTACTAGT'' CCCCATGGCGATGCCTTAAA
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{| class="wikitable" border ="1px solid black"
{| class="wikitable" border ="3px solid black"
|-
|-
|description
|'''Description'''
|primer 1 (mir sense)
|'''Primer 1 (mir sense)'''
|primer 2 (mir antisense)
|'''Primer 2 (mir antisense)'''
|primer 3 (mir* sense)
|'''Primer 3 (mir* sense)'''
|primer 4 (mir* antisense)
|'''Primer 4 (mir* antisense)'''
|-
|-
|[http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Designer&project=stdwmd&rm=show_results&id=7205 GFP]
|[http://wmd3.weigelworld.org/cgi-bin/webapp.cgi?page=Designer&project=stdwmd&rm=show_results&id=7205 GFP]

Revision as of 11:54, 23 June 2010

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Wednesday June 23, 2010

amiRNA

amiRNA pcr diagram
    • Go to the [amiRNA section] for our primers for this multi step PCR or see chart below:


Description Forward (A) Reverse (B)
biobrick RS300 CCTTGAATTCGCGGCCGCATCTAGA CCCACAAACACACGCTCGGA AAGGCTGCAGCGGCCGCTACTAGT CCCCATGGCGATGCCTTAAA


Description Primer 1 (mir sense) Primer 2 (mir antisense) Primer 3 (mir* sense) Primer 4 (mir* antisense)
GFP gaTTAGTGGTCGGCGAGCTGCACtctctcttttgtattcc gaGTGCAGCTCGCCGACCACTAAtcaaagagaatcaatga gaGTACAGCTCGCCGTCCACTATtcacaggtcgtgatatg gaATAGTGGACGGCGAGCTGTACtctacatatatattcct
LTP 1 gaTCTAACTATGTATAGGACCACtctctcttttgtattcc gaGTGGTCCTATACATAGTTAGAtcaaagagaatcaatga gaGTAGTCCTATACAAAGTTAGTtcacaggtcgtgatatg gaACTAACTTTGTATAGGACTACtctacatatatattcct
Bet v1 gaTTGGTTATATATATCGCACCTtctctcttttgtattcc gaAGGTGCGATATATATAACCAAtcaaagagaatcaatga gaAGATGCGATATATTTAACCATtcacaggtcgtgatatg gaATGGTTAAATATATCGCATCTtctacatatatattcct


Transformations

  • Transformed ligations (V0210+LTP1) (V0210+Ger 3 sense) (V0210+Ger 3 antisense)w/turbo cells
  • Grew up colonies w/ RS300 for minipreps


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