User:Dhea Patel/Notebook/Phosphorylation/2012/09/19: Difference between revisions

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#Eight 5mL fractions were collected and spotted on a TLC plate. Using UV, it was determined that fractions 2, 3, and 4 contained ATP.
#Eight 5mL fractions were collected and spotted on a TLC plate. Using UV, it was determined that fractions 2, 3, and 4 contained ATP.
#These fractions were combined and rotovapped.
#These fractions were combined and rotovapped.
#Ruthenium was purified in a silica gel column. Please refer to [[User:Dhea_Patel/Notebook/Phosphorylation/2012/06/25| June 25th's entry]] for procedure.


==Data==
==Data==

Revision as of 07:52, 3 October 2012

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Objective

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Description

  1. 0.0245g ATP (disodium ATP) was measured out. 10mL of 0.1M TEAB was added and the ATP dissolved in the solution.
  2. This solution was run through a column equilibrated to 0.1M TEAB.
  3. Eight 5mL fractions were collected and spotted on a TLC plate. Using UV, it was determined that fractions 2, 3, and 4 contained ATP.
  4. These fractions were combined and rotovapped.
  1. Ruthenium was purified in a silica gel column. Please refer to June 25th's entry for procedure.

Data

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Notes

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