User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/07/25: Difference between revisions
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== | ==Protein Solution Preparation== | ||
* | # Lysozyme | ||
#*M<sub>w</sub> = 14,307 Da | |||
#*0.00637 g in 25 mL vol flask (17.81 μM) | |||
#*0.02538 g in 25 mL vol flask (70.96 μM = 1.01 g/L) | |||
# BSA | |||
#*M<sub>w</sub> = 66,430 Da | |||
#*0.02944 g in 25 mL vol flask (17.73 μM = 1.18 g/L) | |||
# Invertase | |||
#*M<sub>w</sub> = 270,000 Da | |||
#*0.09497 g in 10 mL vol flask (35.2 μM = 9.56 g/L) | |||
#*Did not completely dissolve | |||
<br> | |||
General Procedure for determining appropriate concentration: | |||
*Identify [Au]:[protein] concentration required for fiber formation | |||
*[Au]<sub>f</sub> = 0.25 mM = 250 μM for correct pH | |||
*Want to add 1 mL of each stock, diluted to 10 mL for fiber formation | |||
*[Au]<sub>stock</sub> = 2.5 mM | |||
*For BSA, fiber formation occurs at [Au]:[protein] = 135 | |||
*[Protein] = [Au]/135 = 18.5 μM | |||
*Note that the final prepared solution will have [protein]<sub>f</sub> = 1.85 μM | |||
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