User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/12: Difference between revisions

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==Objective==
==Objective==


<b>Conductivity of AuNP II</b>
To repeat the experiment on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/11 10/11/11] and make electrodes to test conductivity of MBP protein fibers containing AuNPs using a four point probe.
To repeat the experiment on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/11 10/11/11] and make electrodes to test conductivity of MBP protein fibers containing AuNPs using a four point probe.
<b>DNA Sequencing</b>
To compare the mutant GFP DNA sequence purified on [http://openwetware.org/wiki/User:Elaine_Marie_Robbins/Notebook/CHEM-496/2011/10/05 10/05/11] to the wild type GFP DNA sequence to determine if the mutation is present.


==Description==
==Description==

Revision as of 09:49, 12 October 2011

Conductivity of AuNP II, DNA Sequencing <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Conductivity of AuNP II To repeat the experiment on 10/11/11 and make electrodes to test conductivity of MBP protein fibers containing AuNPs using a four point probe.

DNA Sequencing To compare the mutant GFP DNA sequence purified on 10/05/11 to the wild type GFP DNA sequence to determine if the mutation is present.

Description

  1. Glue Pd wire onto Pd foil using conductive epoxy.
  2. Heat with heat gun until epoxy dries.
  3. Repeat with 3 more wires/foils.
  4. Glue wires/foils to Teflon plate with Instant Krazy Glue.

Data

Areas of Pd foil used, from left to right: 35.20 mm2, 41.16 mm2, 40.18 mm2, 38.64 mm2.


NNNNNNNNNNNTTTGTTNNACTTTAAGAAGGAGNTATACATATGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCAT GACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCGATGGGGATCCGAATTCGCCACCATGGTGAGC AAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC CCTCGTGACCACCTTGACCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCC ATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGG GCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAA CTACAACAGCCACAAGGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGACCCGCCACAACATCGAG GACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACC TGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGAT CACTCTCGGCATGGACGAGCTGTACAAGTAACTCGAGAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT GCTGCCACCGCTGANCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACT

Notes