User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/26: Difference between revisions
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# Empty Coomassie Brilliant Blue R250 stain from gels. | # Empty Coomassie Brilliant Blue R250 stain from gels. | ||
# Add destaining solution. | # Add destaining solution. | ||
# Place gels on shaker for | # Place gels on shaker for 2 hr. | ||
# Dump out destaining solution. | # Dump out destaining solution. | ||
# Add more destaining solution. | # Add more destaining solution. | ||
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<b>AuNP VI</b> | <b>AuNP VI</b> | ||
After 30 min in the oven, yellow fibers began to appear at the surface of the gold-containing solution. At 60 min, a greenish-gold solid began to form near the surface of the solution. | |||
The solution containing only BSA and water remained clear throughout the experiment. | |||
<b>SDS-PAGE II</b> | |||
The gels indicated that the desired protein was present, but it was not pure. | |||
<center>[[Image:AllGels.JPG|800px]]</center> | |||
<center>[[Image:Group3.JPG|800px]]</center> | |||
==Notes== | ==Notes== |
Revision as of 10:30, 1 November 2011
AuNP VI, SDS-PAGE II | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAuNP VI To synthesize AuNP using the procedure developed here:
http://pubs.acs.org/doi/abs/10.1021/jp110296y
To run an SDS-PAGE to determine whether or not MBP-intein fusion proteins are being expressed cells comparable to the cells frozen on 09/28/11 and to continue the experiment on 10/25/11. DescriptionAuNP VI
DataAuNP VI After 30 min in the oven, yellow fibers began to appear at the surface of the gold-containing solution. At 60 min, a greenish-gold solid began to form near the surface of the solution. The solution containing only BSA and water remained clear throughout the experiment. SDS-PAGE II The gels indicated that the desired protein was present, but it was not pure.
NotesSDS-PAGE II Protein solutions used were from group 2:
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