User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Effect of Tris Buffer on Protein Fibers</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Objective== | ==Objective== | ||
To synthesize a solution of AuNP, to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31 |01/31/11]], and to determine the effect of tris buffer on protein fibers also synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31 |01/31/11]]. | |||
==Description== | ==Description== | ||
<b>Synthesizing AuNP</b> | |||
# Combine 2 mL of 3.66 mM HAuCl<sub>4</sub> and 6.04 mL of 0.0173 mM BSA in a test tube. | |||
# Dilute to 20 mL with water. | |||
# Place in oven at 80°C. | |||
# Leave solutions in oven for 3 hr. | |||
# Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]] over time. | |||
<b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b> | |||
# Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31| 01/31/12]]. | |||
# Add 2 mL 50 mM tris buffer (pH 10.1) to solid protein fibers (no solution). | |||
# Allow fibers to settle. | |||
# Take UV-vis data of tris/fiber solution. | |||
==Data== | ==Data== | ||
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Over time, the optimum wavelength tends to get shorter. | Over time, the optimum wavelength tends to get shorter. | ||
* Graph of absorbance vs wavelength of the protein fibers with added 50 mM tris buffer (pH 10.1) | |||
<center>[[Image:Absorbance vs wavelength tris 2-15-12.jpg]]</center> | |||
* AuNP solution: | |||
<center>[[Image:Aunp solution.JPG]]</center> | |||
==Notes== | ==Notes== |
Revision as of 11:13, 21 February 2012
Effect of Tris Buffer on Protein Fibers | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo synthesize a solution of AuNP, to continue to take UV-vis data over time of 70 Au/BSA solution synthesized on 01/31/11, and to determine the effect of tris buffer on protein fibers also synthesized on 01/31/11. DescriptionSynthesizing AuNP
Data
Over time, absorbance values increase dramatically.
Over time, the optimum wavelength tends to get shorter.
Notes
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