User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21: Difference between revisions
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==Objective== | ==Objective== | ||
To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/ | To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]], and to synthesize protein fibers. | ||
==Description== | ==Description== | ||
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<b>Synthesizing AuNP</b> | <b>Synthesizing AuNP</b> | ||
# Combine 1 mL of 3.66 mM | # Combine 1 mL of 3.66 mM HAuCl<sub>4</sub> and 1.27 mL of 0.0173 mM BSA in a test tube. | ||
# Dilute to 10 mL with water. | # Dilute to 10 mL with water. | ||
# Place in oven at 75°C. | # Place in oven at 75°C. | ||
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<b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b> | <b>Determining the effect of tris buffer on 166 Au/BSA ratio solution</b> | ||
# Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/ | # Take UV-vis data of 166 Au/BSA ratio solution synthesized on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/01/31 |01/31/12]] and modified on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]]. | ||
# Centrifuge for 10 min at 13,200 rpm | # Centrifuge for 10 min at 13,200 rpm. | ||
# Take UV-vis data of supernatant. | # Take UV-vis data of supernatant. | ||
==Data== | ==Data== | ||
* Graph of absorbance vs wavelength for UV-vis data obtained before and after sample was centrifuged, compared with data obtained on [[User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/15 |02/15/12]]. | |||
<center>[[Image:Absorbance vs wavelength 2-21-12.jpg]]</center> | |||
High absorbance values obtained from sample prior to centrifuging is most likely due to light scattering caused by solid protein fibers. | |||
*Magnified graph of absorbance vs wavelength not including data obtained before centrifuging sample. | |||
<center>[[Image:Absorbance vs wavelength magnified 2-21-12.jpg]]</center> | |||
==Notes== | ==Notes== | ||
The presence of a peak at 500-550 nm after centrifuging indicates that AuNP may be present in solution, even though the solution is a 166 Au/BSA ratio solution. Prior to the addition of the 50 mM tris solution (pH 10.1), all Au particles were associated with the protein fibers, and none were in solution. | |||
[[Category:Course]] | [[Category:Course]] | ||
Revision as of 10:19, 17 April 2012
 Effect of Tris Buffer on Protein Fibers II
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ObjectiveTo take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12, and to synthesize protein fibers. DescriptionSynthesizing AuNP
Data
 High absorbance values obtained from sample prior to centrifuging is most likely due to light scattering caused by solid protein fibers.
 NotesThe presence of a peak at 500-550 nm after centrifuging indicates that AuNP may be present in solution, even though the solution is a 166 Au/BSA ratio solution. Prior to the addition of the 50 mM tris solution (pH 10.1), all Au particles were associated with the protein fibers, and none were in solution.
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