User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
=='''Thursday 29th July 2010'''==
* Insert content here...
Today I digested the pBluescript II KS + plasmid and the cI inverter PCR with EcoRI-HF and PstI for posterior cloning of the inverter into the plasmid as follows:<br />
<br />
Reactive..............1x [ul]<br />
DNA....................20<br />
BSA.......................1<br />
Buffer2..................4<br />
H2O....................11<br />
EcoRI-HF...............2<br />
PstI........................2<br />
Total....................40<br />
For the plasmid I did it twice.<br />
<br />
Then I checked the DNA quantities in an agarose gel for posterior ligation.<br />
<br />
[[Image:IMG 0338.JPG|center|300px]]


1º ladder<br />
2º pBluescript II KS +  plasmid<br />
3º pBluescript II KS +  cut with EcoRI-HF and PstI<br />
4º pBluescript II KS +  cut with EcoRI-HF and PstI<br />
5º cI inverter PCR cut with EcoRI-HF and PstI<br />
<br />


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Revision as of 12:05, 30 July 2010

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Thursday 29th July 2010

Today I digested the pBluescript II KS + plasmid and the cI inverter PCR with EcoRI-HF and PstI for posterior cloning of the inverter into the plasmid as follows:

Reactive..............1x [ul]
DNA....................20
BSA.......................1
Buffer2..................4
H2O....................11
EcoRI-HF...............2
PstI........................2
Total....................40
For the plasmid I did it twice.

Then I checked the DNA quantities in an agarose gel for posterior ligation.

1º ladder
2º pBluescript II KS + plasmid
3º pBluescript II KS + cut with EcoRI-HF and PstI
4º pBluescript II KS + cut with EcoRI-HF and PstI
5º cI inverter PCR cut with EcoRI-HF and PstI


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