User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/30: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | =='''Friday 30th July 2010'''== | ||
The plasmid digestions are not suitable for ligation, one is degrading the plasmid so I should throw it away but I’ll do it just in case, the other is partial. For both I raised the volume up to 50 ul and left it to cut all weekend!<br /> | |||
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Reactive.............1x [ul]<br /> | |||
DNA..................---<br /> | |||
BSA...................---<br /> | |||
Buffer2..............+ 2<br /> | |||
H2O..................+ 14<br /> | |||
EcoRI-HF..........+ 2<br /> | |||
PstI...................+ 2<br /> | |||
Total..................50<br /> | |||
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Revision as of 15:46, 30 July 2010
 iGEM Project name 1
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
Friday 30th July 2010The plasmid digestions are not suitable for ligation, one is degrading the plasmid so I should throw it away but I’ll do it just in case, the other is partial. For both I raised the volume up to 50 ul and left it to cut all weekend! | |
