User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/01: Difference between revisions
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==Gel of PCR and digestion== | ==Gel of PCR and digestion== | ||
*1 Gene'O Mixed Ruler | *1 Gene'O Mixed Ruler | ||
*2 Paz's PCR | *2 Paz's PCR ~1Kbp as expected | ||
*3 Tube 1 PCR | *3 Tube 1 PCR ~1Kbp as expected | ||
*4 Tube 2 PCR | *4 Tube 2 PCR ~1Kbp as expected | ||
*5 Dobuble Digestion | *5 Dobuble Digestion ~3.4Kbp as expected | ||
[[Image:PCR.Prom Km.B0015.1Aug2011.JPG|200px]] | [[Image:PCR.Prom Km.B0015.1Aug2011.JPG|200px]] | ||
Status: Success!!! | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 22:32, 1 August 2011
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Objectives
Digestion
PCR for Kanamycin
Denaturation step: 30seg 94°C Annealing step: 30seg 55°C Extension/elongation step: 40seg 72°C Final elongation 30seg 72°C Final hold: 3hrs 4°C Gel of PCR and digestion
Status: Success!!!
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