User:Klare Lazor/Notebook/Chem-496-001/2012/02/01: Difference between revisions
From OpenWetWare
Klare Lazor (talk | contribs) (→Data) |
Klare Lazor (talk | contribs) |
||
Line 26: | Line 26: | ||
*BSA/AuNP Preparation of 60:1 based off stocks above | *BSA/AuNP Preparation of 60:1 based off stocks above | ||
**In oven for four hours | **In oven for four hours | ||
Calculations for dilutions of 60:1 in 10 mL | |||
*(1mLBSA)(0.0000015mols/L)= (10mL)(x) | |||
**x=0.00000015mols/L of BSA in 10mL of water total volume | |||
*(60)/(1)=(x)/(0.00000015mols/LBSA) | |||
**x=0.000009mols/L of HAuCl4 | |||
*(x)(0.0027mols/L)=(10mL)/(0.000009mols/L) | |||
**x= 0.033mL of HAuCl5 in 10mL of water total volume | |||
So, | |||
33µL of HAuCl4 | |||
1000µL of BSA | |||
8967µL of H20 | |||
Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1 | |||
==Data== | ==Data== |
Revision as of 10:07, 5 February 2012
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Description
Calculations for dilutions of 60:1 in 10 mL
So, 33µL of HAuCl4 1000µL of BSA 8967µL of H20 Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1 Data
Notes
|