User:Klare Lazor/Notebook/Chem-496-001/2012/02/06

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Objective

  • remake solutions to determine what is going wrong

Description

  • Calculations for dilutions of 60:1 in 10 mL
  • Stock Solutions
    • HAuCl4 in 50mL solution
      • 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
      • 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
    • BSA in 50mL solutions
      • 1.57µM=0.000015mols/L x 0.05L = 7.5 E-7 mols
      • 7.5 E-7 mols x 66,000 g/mol = 0.0495 grams of BSA


  • BSA/AuNP Preparation of 60:1 based off stocks above
    • In oven for four hours


Calculations for dilutions of 60:1 in 10 mL

  • (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
    • x=0.000245mols/L of HAuCl4 in 10mL of water total volume
  • (60)/(1)=(0.000245)/(x)
    • x=0.000004 mols/L of BSA
  • (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
    • x= 2.666 mL of BSA in 10mL of water total volume


  • So,
    • 2666 µL of bsa
    • 906 µL of HAuCl4
    • 6428 µL of H20

Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1

Data

  • finally realized bsa stock solution was to dilute remade solutions today with the correct
  • purple solution resulted

Notes

This area is for any observations or conclusions that you would like to note.


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