Objective
 remake solutions to determine what is going wrong
Description
 Calculations for dilutions of 60:1 in 10 mL
 Stock Solutions
 HAuCl4 in 50mL solution
 2.7mM= 0.0027mols/L x 0.05 L = 1.35E4 mols HAuCl4
 1.35E4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
 BSA in 50mL solutions
 1.57µM=0.000015mols/L x 0.05L = 7.5 E7 mols
 7.5 E7 mols x 66,000 g/mol = 0.0495 grams of BSA
 BSA/AuNP Preparation of 60:1 based off stocks above
Calculations for dilutions of 60:1 in 10 mL
 (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
 x=0.000245mols/L of HAuCl4 in 10mL of water total volume
 (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
 x= 2.666 mL of BSA in 10mL of water total volume
 So,
 2666 µL of bsa
 906 µL of HAuCl4
 6428 µL of H20
Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1
Data
 finally realized bsa stock solution was to dilute remade solutions today with the correct
 purple solution resulted
Notes
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