User:Klare Lazor/Notebook/Chem-496-001/2012/02/01: Difference between revisions

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*synthesize AUNP at a concentration of 60 HAUCl4 to 1 BSA
*synthesize AUNP at a concentration of 60 HAUCl4 to 1 BSA
*prepare stock solutions


==Description==
==Description==
* Stock Solutions
** HAuCl4 in 50mL solution
*** 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
*** 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
** BSA in 50mL solutions
***1.5µM=0.000015mols/L  x 0.05L  = 7.5 E-7 mols
***7.5 E-7 mols  x  66,000 g/mol  =  0.0495 grams of BSA


==Data==
* Add data and results here...


==Notes==
*BSA/AuNP Preparation of 60:1 based off stocks above
This area is for any observations or conclusions that you would like to note.
**In oven for four hours
 
 
 
Calculations for dilutions of 60:1 in 10 mL
*(0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
**x=0.000245mols/L of HAuCl4 in 10mL of water total volume
 
*(60)/(1)=(0.000245)/(x)
**x=0.000004 mols/L of BSA


*(x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
**x= 2.666 mL of BSA in 10mL of water total volume


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Miscellaneous]]


*So,
**2666 µL of HAuCl4
**906 µL of BSA
**6428 µL of H20


[[Category:Course]]
Check on ratio of concentration=  (0.000009mols/L)/ (0.00000015mols/LBSA) =  60/1
[[Category:Miscellaneous]]


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
==Data==
|}
*nothing, bsa stock was to dilute to produce any nano particles


__NOTOC__
==Notes==
*No nano particles were made due to lack of HAuCl4.

Revision as of 11:18, 29 February 2012

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Objective

  • synthesize AUNP at a concentration of 60 HAUCl4 to 1 BSA
  • prepare stock solutions

Description

  • Stock Solutions
    • HAuCl4 in 50mL solution
      • 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
      • 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
    • BSA in 50mL solutions
      • 1.5µM=0.000015mols/L x 0.05L = 7.5 E-7 mols
      • 7.5 E-7 mols x 66,000 g/mol = 0.0495 grams of BSA


  • BSA/AuNP Preparation of 60:1 based off stocks above
    • In oven for four hours


Calculations for dilutions of 60:1 in 10 mL

  • (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
    • x=0.000245mols/L of HAuCl4 in 10mL of water total volume
  • (60)/(1)=(0.000245)/(x)
    • x=0.000004 mols/L of BSA
  • (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
    • x= 2.666 mL of BSA in 10mL of water total volume


  • So,
    • 2666 µL of HAuCl4
    • 906 µL of BSA
    • 6428 µL of H20

Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1

Data

  • nothing, bsa stock was to dilute to produce any nano particles

Notes

  • No nano particles were made due to lack of HAuCl4.
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