User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> UV-vis Measurements & Sample Preparation</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Date==
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==October 2, 2013==
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*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
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*Sample preparation based on calculated concentrations
===Procedures===
===Procedures===
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===Data===
===Data===
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=====Absorbance Measurements from 10/25/2013=====
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[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
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Beer's Law calculations:<br.>
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[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]
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=====Absorbance Measurements, Today=====
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I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
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<br.>
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[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
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Beer's Law calculations:<br.>
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[[Image:2013_1002_ZnPPIX_calcs.PNG]]
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===Notes===
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=====Samples Prepared=====
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*2.47uM ZnPPIX
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*2.47uM ZnPPIX/1.11uM DNA-SH
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*2.47uM ZnPPIX/1.11uM DNA (4)
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*2.47uM ZnPPIX/1.11uM DNA (8)
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*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
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*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
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*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
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*2.47uM ZnPPIX/11.75nM AuNPs
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===Notes===
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*Run today's samples for absorbance and fluorescence
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*Ask Dr Hartings for more ZnPPIX
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Revision as of 11:23, 2 October 2013

UV-vis Measurements & Sample Preparation Main project page
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October 2, 2013

  • Absorbance measurements and Beer's law calculations for samples from 10/25/2013
  • Sample preparation based on calculated concentrations

Procedures

Data

Absorbance Measurements from 10/25/2013

Image:2013_1002_ZnPPIX_from_last_week.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG

Absorbance Measurements, Today

I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
Image:2013_1002_ZnPPIX_for_calcs.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_calcs.PNG

Samples Prepared
  • 2.47uM ZnPPIX
  • 2.47uM ZnPPIX/1.11uM DNA-SH
  • 2.47uM ZnPPIX/1.11uM DNA (4)
  • 2.47uM ZnPPIX/1.11uM DNA (8)
  • 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
  • 2.47uM ZnPPIX/11.75nM AuNPs

Notes

  • Run today's samples for absorbance and fluorescence
  • Ask Dr Hartings for more ZnPPIX


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