User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09

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(Samples Prepared)
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==October 2, 2013==
 
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===Procedures===
 
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*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
 
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*Sample preparation based on calculated concentrations
 
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===Data===
 
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=====Absorbance Measurements from 10/25/2013=====
 
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[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
 
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Beer's Law calculations:<br.>
 
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[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]
 
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=====Absorbance Measurements, Today=====
 
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I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
 
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<br.>
 
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[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
 
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Beer's Law calculations:<br.>
 
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[[Image:2013_1002_ZnPPIX_calcs.PNG]]
 
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=====Samples Prepared=====
 
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"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
 
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*2.47uM ZnPPIX
 
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*2.47uM ZnPPIX/1.11uM DNA-SH
 
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*2.47uM ZnPPIX/1.11uM DNA (4)
 
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*2.47uM ZnPPIX/1.11uM DNA (8)
 
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*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
 
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*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
 
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*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
 
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*2.47uM ZnPPIX/11.75nM AuNPs
 
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===Notes===
 
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*Run today's samples for absorbance and fluorescence
 
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*Ask Dr Hartings for more ZnPPIX
 
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Revision as of 10:35, 2 October 2013

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