User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23: Difference between revisions
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(New page: * PCR ** Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4) *** 6His Tag: 6His_Tag-F and [[User:Mbennie/Not...) |
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Revision as of 10:33, 22 January 2008
- PCR
- Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4)
- 6His Tag: 6His_Tag-F and 6His_Tag-R
- FLAG Tag: FLAG_Tag-F and FLAG_Tag-R
- Myc Tag: Myc_Tag-F and Myc_Tag-R
- HA Tag: HA_Tag-F and HA_Tag-R
- GCN4 Leucine Zipper: GCN4-F and GCN4-R2
- Same protocol as 1.22.2008
- Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4)
