User:Samiye Yaman/Notebook/Lab 581/2012/10/24: Difference between revisions

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==Objective==


==Description==
==Description==
*The course is designed by American University to experiment different aspects of PVOH films.


*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
'''Part 1''' '''(ISE)'''
 
*0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
 
*Channel 1 of the Orien Versa Star was used.
 
*Before each measurement 30 sec was wait.
 
 
'''Part 2''' '''Preparation of Porphyrin dilutions'''
 
*Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
 
*Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
 
*Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
 
*Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
 
*Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)
 
==Data==
 
'''ISE results for sodium sulfate dilutions'''
 
*Dilution 1(1.25ppm)= -180mV
 
*Dilution 2(2.5ppm)=  -182.6mV
 
*Dilution 3(5ppm)  = -182.4mV
 
*Dilution 4(10ppm )= -177.4mV
 
*Dilution 5(20ppm)= -174.6mV
 
 
*We decided detection limit was between dilution 1 and 2.
 
*More negative number means less sodium ion in the solution.
 
*The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.
 
==Notes==
This area is for any observations or conclusions that you would like to note.
 
 
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
 
[[Category:Course]]
[[Category:Miscellaneous]]
 
 
 
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*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
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Revision as of 20:36, 30 October 2012

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Objective

Description

Part 1 (ISE)

  • 0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
  • Channel 1 of the Orien Versa Star was used.
  • Before each measurement 30 sec was wait.


Part 2 Preparation of Porphyrin dilutions

  • Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
  • Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
  • Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
  • Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
  • Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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