User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/16: Difference between revisions

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* Treated membrane with secondary antibody and obtained
* Treated membrane with secondary antibody and obtained
** a lot of unspecicific binding on membrane
** a lot of unspecicific binding on membrane
 
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Revision as of 01:27, 11 June 2010

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Split cells for ELISA and secondary antibody Immunoblot <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • 24-wells (6x 750.000 cells per well)& 10cm dishes (8x 1000000 cells per plate) plated out (D9 P24)
    • probably too little cells on 24-wells due to miscalculation
  • Treated membrane with secondary antibody and obtained
    • a lot of unspecicific binding on membrane