User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/15

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Summary

Materials & Methods

Materials

Method

  • 8% (2x) and 15% (1x) gels were prepared (14April2010 as described 01March2010
  • Gels were run and transferred as described 09April2010
  • Membranes were treated with Ponceau S staining and blocked in 5% BSA TBST solution for 1 h @ RT
  • Antibodies used on membranes
    • AKAP7(Rabbit polyclonal antibody) (Abnovation)
      • 15% gel membrane (below 25 kDa)
    • GSKIP (Rabbit) (In House)
      • Lower half of 15% gel membrane (below 25 kDa)
    • AKAP-Lbc (Goat polyclonal IgG) (Santa Cruz Biotechnology)
      • 8%
    • AKAP18δ5 (Rabbit) (In House)
      • 15%
    • AKAP95 (Mouse) (Transuction Laboraties)
      • 8%
    • AKAP12 (Sheep polyclonal) (Abcam)
      • 8%
    • AKAP450 (Mouse) BD Transduction
      • 8%
    • AKAP1 (Rabbit) (Bethyl)
      • 8%
    • mAKAP (Rabbit)(Upstate)
      • 8%
    • AKAP79 (Rabbit)(Upstate)
      • Upper half of 15% gel membrane (above 25 kDa)

Notes

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