User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/09/22: Difference between revisions
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==Summary== | ==Summary== | ||
* | * Dilution of samples to 20 μg/30 μL (5x) + 10 μL LB per 30 μL sample | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''μL''' | |||
| align="center" style="background:#f0f0f0;"|'''μL''' | |||
|- | |||
| ||Sample||ddH2O | |||
|- | |||
| Jurkat||25,0||125,0 | |||
|- | |||
| HEK293 (12/08/2010)||25,4||124,6 | |||
|- | |||
| HeLa -S (p17 (20.6.2010)||10,7||139,3 | |||
|- | |||
| S0 (7.5.2010)||97,9||52,1 | |||
|- | |||
| S1||21,5||128,5 | |||
|- | |||
| S2||62,0||88,0 | |||
|- | |||
| S3||22,2||127,8 | |||
|- | |||
| C0 (7.5.2010)||150,0||0,0 | |||
|- | |||
| C1||24,6||125,4 | |||
|- | |||
| C2||14,9||135,1 | |||
|- | |||
| C3||43,9||106,1 | |||
|} | |||
* Run 1x 8% SDS-PAGE | |||
{| border="1" | |||
|+'''8% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010''' | |||
! align="center" style="background:#f0f0f0;"|# | |||
! align="center" style="background:#f0f0f0;"|<u>1</u> | |||
! align="center" style="background:#f0f0f0;"|<u>2</u> | |||
! align="center" style="background:#f0f0f0;"|<u>3</u> | |||
! align="center" style="background:#f0f0f0;"|<u>4</u> | |||
! align="center" style="background:#f0f0f0;"|<u>5</u> | |||
! align="center" style="background:#f0f0f0;"|<u>6</u> | |||
! align="center" style="background:#f0f0f0;"|<u>7</u> | |||
! align="center" style="background:#f0f0f0;"|<u>8</u> | |||
! align="center" style="background:#f0f0f0;"|<u>9</u> | |||
! align="center" style="background:#f0f0f0;"|<u>10</u> | |||
! align="center" style="background:#f0f0f0;"|<u>11</u> | |||
! align="center" style="background:#f0f0f0;"|<u>12</u> | |||
! align="center" style="background:#f0f0f0;"|<u>13</u> | |||
! align="center" style="background:#f0f0f0;"|<u>14</u> | |||
! align="center" style="background:#f0f0f0;"|<u>15</u> | |||
|- | |||
!Gel<br>Lysates | |||
| align="center"|X | |||
| align="center"|Jurkat<br>(30 μL) | |||
| align="center"|HEK293<br>(30 μL) | |||
| align="center"|HeLa -S<br>(30 μL) | |||
| align="center"|Marker<br> (5 μL) | |||
| align="center"|S0<br>(30 μL) | |||
| align="center"|S1<br>(30 μL) | |||
| align="center"|S2<br>(30 μL) | |||
| align="center"|S3<br>(30 μL) | |||
| align="center"|Marker<br> (5 μL) | |||
| align="center"|C0<br>(30 μL) | |||
| align="center"|C1<br>(30 μL) | |||
| align="center"|C2<br>(30 μL) | |||
| align="center"|C3<br>(30 μL) | |||
| align="center"|X | |||
|- | |||
|} | |||
* Run at 200 V for 40 min. | |||
<!-- | <!-- | ||
==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 05:19, 22 September 2010
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