BME100 f2013:W900 Group12 L2
From OpenWetWare
BME 100 Fall 2013  Home People Lab WriteUp 1  Lab WriteUp 2  Lab WriteUp 3 Lab WriteUp 4  Lab WriteUp 5  Lab WriteUp 6 Course Logistics For Instructors Photos Wiki Editing Help  
OUR TEAMLAB 2 WRITEUPDescriptive StatisticsExperiment 1 Human Study Descriptive Statistics
Experiment 2 Rat Study Descriptive Statistics
ResultsExperiment 1
Experiment 2
AnalysisExperiment 1 Using the ANOVA test for the effect of LPS doses on Inflammotin levels in humans, we found that the pvalue between groups was 1.4 x 10^16, proving to be far below our .05 significance level. We can thus safely conclude that there is a significant statisical difference in Inflammotin levels between the groups of humans given different doses of the LPS drug.
Using the TTest to determine the effect of LPS doses on Inflammotin levels in rats, we discovered that our Pvalue is 0.867403, which is greater than 0.05, which is not significant. Because of this there is no statistical difference in Inflammotin levels between the two groups of rats that were given different doses of the LPS drug.
Summary/DiscussionIn Experiment One we observed the effect different doses of the inflammation inducing agent lipopolysaccharide (LPS) had on the amount of Inflammotin in the blood of humans. Through ANOVA, we determined there is indeed a statistically significant difference among all the dosages. This therefore provides support for our anticipated conclusion that increasing LPS doses increases the amount of inflammotin in the blood. In Experiment Two, in which inflammotin was measured in rats, the ELISA test revealed results that were not statistically significant. After performing a two sample ttest assuming equal variance, we found our pvalue to be much higher than the accepted significance cutoff of 0.05. This evidence contradicts Kristen's assumption that LPS treatment in the rats significantly increased inflammotin levels. In conclusion, according to our study, LPS treatment in human subjects is a valid way to significantly increase inflammotin levels in the blood. In rats, however, LPS does not prove to be an effective treatment.
