BME100 f2017:Group7 W0800 L5
BME 100 Fall 2017 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPPCR Reaction ReportThe pipetting of the samples was successful because there was little to no remaining liquid in the plastic test tubes. The pre-lab readings were extremely helpful because it introduced all of the concepts from this experiment before actually participating in the investigation. The pipetting simulation gave insight to the first and second stops for the pipette; the first being for extracting the liquid and the second for expelling of the liquid. All of the final reaction vials had the same amount of liquid indicating that the contents were added accurately. The labeling scheme consists of a G7 on both separate test tube racks and the patient and sample number on each individual test tube. For example, patient 1 sample 2 would be indicated '1-2.' Fluorimeter ProcedureImaging set-up First, our fluorimeter set up consisted of placing the fluorimeter on a level surface surrounded by a black box to eliminate as much light as possible. The fluorimeter was also placed on top of two test tube boxes to level the iPhone 6 camera with the drop of sample. The iPhone 6 was stabilized by a phone holder which was placed 6cm away from the fluorimeter. When the set up was complete, there was complete darkness within the black box with the exception of the blue light(~470nm wavelength) emitted by the fluorimeter.
Placing Samples onto the Fluorimeter
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA The following images are of the high concentration (5 μg/mL), low concentration (0.5 μg/mL), and the water droplet respectively. Each image had the color channels split and only the green channel was used to gather data. 5 μg/mL concentration .5 μg/mL concentration Zero concentration
Calibration Curve 1 Calibration Curve 2 Images of Our PCR Negative and Positive Controls The following images were taken of the positive and negative controls respectively. They are taken from a green color channel that was split using ImageJ.
PCR Results: Summary
The negative concentration observed for the negative control above could be due to an error found in the calibration curves. However, the difference between the positive and negative concentrations support the validity of this experiment.
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