Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/26

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02/26/2015 Since the sequencing results failed, we decided to troubleshoot and find out whether our error is with the primers or the plasmid. I performed a digestion again with XbaI and PstI. I created a reaction for each plasmid sample that I mini prepped on Monday. Reactions are as follows: Thing Vol 0.25ug DNA sample 7.5 uL XbaI 0.5 uL PstI 0.5 uL Green digest buffer 2 uL Water 9.5 uL After pipetting contents into each tube, I incubated the reactions for 15 minutes at 37°C. The next step is to run a gel with each reaction and see if we get the desired results.