IGEM:Brown/2008/Notebook/Team Resistance/2008/06/11

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Team Resistance

  • Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Testing apparatus with NaCl at different concentrations

• 3 plates with 0.05M NaCl (0.02922g in 10mL)

• 3 plates with 0.005M NaCl (0.02922g in 100mL)

• 3 plates with 0.001M NaCl (0.01461g in 250mL)

dilute to each concentration then put 10 mL of each solution in respective plates

Control: distilled water

Results:

Control: 4.108Mohm, 2.517Mohm, 2.934Mohm

0.001M NaCl: 393.7 kohm, 265.5kohm, 579.6kohm

0.005M NaCl: 264.9kohm, 266kohm, 326.1kohm

0.05M NaCl: 233.5kohm, 46.69kohm, 97.1kohm

control 0.001M NaCl 0.005M NaCl 0.05M NaCl

3.186MΩ 0.4129MΩ 0.2857MΩ 0.1258MΩ

0.001M NaCl: 2.7731MΩ change

0.005M NaCl: 2.9003MΩ change

0.05M NaCl: 3.0602MΩ change

• Our wires were not long enough to touch the solution in the plates—next time don’t tape wires in place until right before experiment begins

• WE NEED A COMPUTER BASED VOLTMETER


Questions for Dan Ludwig (Meeting Friday 3:00pm)

Should touching the plastic significantly change the resistance?

What if distance changes slightly?

All considerations of our apparatus

Any suggestions for a real-time, computer based voltmeter?

Tomorrow: get plates from Harvard lab!

Create glycerol stocks of the plasmids




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